Transformed (Simian Virus 40) African Green Monkey Kidney Fibroblast Cells
COS-1 is a transformed cell line that was developed by Yakov Gluzman from the CV-1 line. Two other similarly transformed lines, COS-3 and COS-7, were also initiated from the CV-1 line by Gluzman.
The COS-1 cell line was produced via transformation of the previously established line with an origin defective mutant of simian virus 40 (SV40) that codes for wild type large tumor antigen (T antigen).
Integrated into COS-1 cells is a copy of the entire early region of the SV40 genome.
The transformed African green monkey kidney cells presented in the digital image above were resident in an adherent culture immunofluorescently labeled with Alexa Fluor 488 and Alexa Fluor 568 conjugated to goat secondary antibodies that target mouse anti-fibrillarin (nucleoli) and rabbit anti-giantin (targeting the Golgi complex) primary antibodies, respectively.
Nuclei were stained with Hoechst 33258. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks.
During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.
Transformed (Simian Virus 40) African Green Monkey Kidney Fibroblast Cells (COS-1 Line)
The intracellular relationship between mitochondria and the filamentous actin network was visualized in a culture of COS-1 fibroblasts (illustrated above) with MitoTracker Red CMXRos and Alexa Fluor 488 conjugated to phalloidin.
Cell nuclei were counterstained with Hoechst 33342. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks.
During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.
Transformed (Simian Virus 40) African Green Monkey Kidney Fibroblast Cells (COS-1 Line)
Presented in the digital image above is an adherent culture of COS-1 fibroblasts that was labeled with Alexa Fluor 488 conjugated to soybean agglutinin, a lectin that selectively binds terminal alpha- and beta-N-acetylgalactosamine and galactopyranosyl residues.
In addition, the cells were labeled with Texas Red conjugated to phalloidin and DAPI, targeting the cytoskeletal F-actin network and DNA, respectively.
Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks.
During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.
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