Leading platform for advanced imaging
The Eclipse Ti2 inverted microscope delivers an unparalleled 25mm field of view (FOV) that revolutionizes the way you see. With this incredible FOV, the Ti2 maximizes the sensor area of large-format CMOS cameras without making compromises, and significantly improves data throughput. The Ti2's exceptionally stable, drift-free platform is designed to meet the demands of super-resolution imaging while its unique hardware-triggering capabilities enhance even the most challenging, high-speed imaging applications. Furthermore, the Ti2's unique, intelligent functions guide users through imaging workflows by gathering data from internal sensors, eliminating the possibility of user errors. In addition, the status of each sensor is automatically recorded during acquisition, providing quality control for imaging experiments and enhancing data reproducibility.
In combination with Nikon's powerful acquisition and analysis software, NIS-Elements, the Ti2 is a total innovation in imaging.
Groundbreaking FOV
As research trends evolve towards large-scale, systems-level approaches, there is an increasing demand for faster data acquisition and higher throughput capabilities. Development of large-format camera sensors and improvements in the data processing capabilities of PCs have facilitated such research trends. The Ti2, with its unprecedented 25mm field of view, provides the next level of scalability, enabling researchers to truly maximize the utility of large-format detectors and future-proof their core imaging platform as camera technologies continue to develop at a rapid pace.
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Conventional FOV 18mm
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New Ti2 FOV 25mm
Bright illumination over a wide area
High-power LEDs deliver bright illumination across the Ti2's large field of view, ensuring clear, consistent results from demanding applications such as high-magnification DIC. Incorporation of a fly-eye lens design provides uniform illumination from edge to edge for quantitative high-speed imaging and seamless tiling of images in stitching applications.
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High-power LED illuminator
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Built-in fly-eye lens
A compact epi-fluorescence illuminator designed for large FOV imaging is equipped with a quartz fly-eye lens and provides high transmittance across a broad spectrum, including UV. Large diameter fluorescence filters with hard coatings deliver large FOV images with a high signal-to-noise ratio.
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Large FOV epi-fl illuminator
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Large diameter fluorescence filter cubes
Objectives for large FOV imaging
Objectives with superior image flatness ensure high quality images from edge to edge. Utilizing the maximum potential of the OFN25 objective significantly accelerates data collection.
Large diameter observation optics
The diameter of the observation light path has been enlarged in order to achieve a field number of 25 at the imaging port. The resulting large FOV is capable of capturing approximately double the area of conventional optics, enabling users to gain maximum performance from large-format sensors such as CMOS detectors.
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Enlarged tube lens
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Imaging port with large 25 field number
Cameras for large-volume data acquisition
The DS-Qi2 high-sensitivity monochrome camera and DS-Ri2 high-speed color camera are equipped with large 36.0 x 23.9 mm, 16.25 megapixel CMOS image sensors, enabling maximum performance with the Ti2's large 25mm FOV.
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D-SLR camera technology optimized for microscopy
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Apodized phase contrast
Nikon's unique apodized phase contrast objectives with selective amplitude filters dramatically increase contrast and reduce halo artifacts to provide detailed high-definition images.
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Apodizedphase plate is incorporated in APC objectives
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BSC-1 cells captured with CFI S Plan Fluor ELWD ADM 40XC objective
DIC (Differential Interference Contrast)
Nikon's highly-regarded DIC optics provide uniformly clear and detailed images with high resolution and contrast throughout the magnification range. DIC prisms are individually tailored for each objective lens to provide the highest-quality DIC images for every sample.
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DIC prisms matched to individual objectives are mounted in the nosepiece
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DIC and epi-fluorescence images:
25mm FOV image of neurons (DAPI, Alexa Fluor® 488, Rhodamine-Phall
NAMC (Nikon Advanced Modulation Contrast)
This is a plastic-compatible, high-contrast imaging technique for unstained, transparent samples such as oocytes. NAMC provides pseudo-three-dimensional images with a shadow-cast appearance. The direction of contrast can be easily adjusted for each sample.
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NAMC objective lenses contain rotatable modulators
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NAMC image:
Mouse embryos, captured with CFI S Plan Fluor ELWD NAMC 20XC objective
Volume Contrast [Ti2-E]
Volume Contrast technique utilizes a series of label-free, brightfield images captured at various Z-depths to assemble a phase distribution image.
Volume Contrast renders cells easily identifiable as objects for automated counting and area analysis. As this method utilizes brightfield imaging, Volume Contrast enables in-line, non-destructive analysis of cells, suitable for a wide variety applications. Note. For Ti2-E only.
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Brightfield
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Volume Contrast
Features of Volume Contrast（VC)
Accurate identification of cells from label-free cultures for automated cell counting and area measurements.
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Cell identification based on phase contrast images
Cells encircled in red are incorrectly identifie -
Cell identification using VC images
Cells encircled in red are correctly identified as three
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Negligible Meniscus Effect on Cell Identification
Phase contrast images are negatively impacted at the edges of wells due to the meniscus effect. Volume Contrast bypasses this effect and enables cells at the edges of wells to be clearly identified, resulting in increased cell counts and improved statistics.
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Phase contrast image
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VC image
Focus perfected
Mechanically redesigned for ultra-high stability
In order to improve the focusing stability, both Z-drive and PFS autofocusing mechanisms have been completely re-designed.
The new Z-focusing mechanism is smaller and positioned adjacent to the nosepiece to minimize vibrations. It remains adjacent to the nosepiece even in an expanded (staged-up) configuration, ensuring stability for all applications.
① High stability Z-focusing mechanism remains adjacent to the nosepiece even in expanded configurati
The detector portion of the Perfect Focus System (PFS) has been detached from the nosepiece in order to reduce mechanical load on the objective nosepiece. This new design also minimizes heat transfer, which contributes to a more stable imaging environment. Towards this end, the power consumption of the Z-drive motor has also been reduced. Combined, these mechanical redesigns result in an ultra-stable imaging platform, perfectly suited for single-molecule imaging and super-resolution applications.
① PFS nosepiece
② PFS Measuring Unit
Real time focus correction with the PFS: Simply perfect
The Perfect Focus System (PFS) automatically corrects focus drift caused by temperature changes and mechanical vibrations, which can be caused by a variety of factors including the addition of reagents to the sample and multi-position imaging.
The PFS maintains focus by detecting and tracking the position of the coverslip surface in real time. Unique optical offset technology allows users to easily maintain focus at a desired position offset from the cover slip surface. The PFS automatically and continuously maintains focus by means of a built-in linear encoder and high speed feedback mechanism, providing highly reliable images even during long-term, complex imaging tasks.
PFS is compatible with a wide range of applications, from routine experiments involving plastic culture dishes to single-molecule imaging and multi-photon imaging. It is also compatible with a wide range of wavelengths, from ultraviolet to infrared, meaning it can be used for multi-photon and optical tweezer applications.
Intuitive operation
The Ti2 has been completely redesigned, from the overall body design to the selection and placement of every button and switch, for the ultimate in user experience. The controls are easy to use even in the dark, where the majority of imaging experiments are conducted. The Ti2 provides an intuitive and effortless user interface so researchers can focus on the data and not on microscope control.
Thoughtfully designed layout for microscope control
The placement of all of the buttons and switches are based on the type of illumination they control. Buttons that control diascopicobservation are positioned on the left side of the microscope and those that control epi-fluorescence observation are on the right side. Buttons that control common operations are on the front panel. This use of zoning provides an easy-to-remember layout, a desirable feature when operating the microscope in a dark room.
① Shuttle switch (Ti2-E)
Shuttle switches have been incorporated into the design to control devices such as the fluorescence filter turret and objective nosepiece. These types of switches emulate the feel of manually rotating these devices, for intuitive control. Additional functionality can be incorporated into these shuttle switches so that a single switch can operate multiple related devices. For example, the shuttle switch for the fluorescence filter turret not only rotates the turret but also opens and closes the fluorescence shutter when the user presses the switch. It is also possible to program these switches to operate a barrier filter wheel and the external phase contrast unit.
② Programmable Function button (Ti2-E/A)
Conveniently located Function buttons allow customization of the user interface. Users can select from more than 100 functions, including control of motorized devices such as shutters and even signal output to external devices via the I/O port for triggered acquisition. Mode functions, which enable instant changing of observation methods by storing the settings of each motorized device, can also be assigned to these buttons.
③ Focusing knob (Ti2-E)
A focus acceleration button and a PFS engagement button are provided adjacent to the focusing knobs. The two buttons are easily identified by touch because of their different shapes. Focusing speed is automatically adjusted for the objective in use, enabling stress-free operation by maintaining an ideal focusing speed.
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