Tenfold increased resolution in x, y and z.
Tochastic Optical Reconstruction Microscopy (STORM) reconstructs a super-resolution fluorescent image by combining precise localization information for individual fluorophores in complex fluorescent microscope specimens. N-STORM takes advantage of Nikon's powerful Ti2-E inverted microscope and applies high-accuracy, multi-color localization and reconstruction in three dimensions (xyz) to enable super-resolution imaging at tenfold the resolution of conventional light microscopes (up to approximately 20 nm in xy).
This powerful technology enables the visualization of molecular interactions at the nanoscopic level, opening up new worlds of scientific understanding.
Tenfold improvement of axial resolution up to 50 nm
In addition to lateral super-resolution, N-STORM utilizes proprietary methods to achieve a tenfold enhancement in axial resolution over conventional light microscopes and provide nanoscale information in 3D.
The 3D-Stack function allows multiple 3D STORM images from different Z positions to be captured and stitched into one image to create thicker STORM images.
Tenfold improvement of lateral resolution up to 20nm
N-STORM utilizes high accuracy localization information for thousands of individual fluorophores present in a field of view to create breathtaking "super-resolution" images, exhibiting spatial resolution that is 10 times greater than conventional optical microscopes.
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Human cervical cancer cells (HeLa S3) labeled with Alexa Fluor ® 647 (NUP153) and ATTO 488 (TPR)
Photos courtesy of: Dr. Michael W. Davidson, National High Magnetic Field Laboratory, Florida State Universi -
Multi-color imaging capability
Multi-color super-resolution imaging can be carried out using both activator-reporter pairs for sequential activation imaging and activator-free labels for continuous activation imaging. This flexibility allows users to easily gain critical insights into the localization and interaction properties of multiple proteins at the molecular level.
High definition, high density images
Newly developed excitation optics and improved image acquisition rates provide increased molecule localization density, resulting in clearer images of macromolecular structures.
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Left: Improved image quality, Right: Before improvement
Scale bar: 5µm
Super-resolution image quality is significantly improved in the same imaging time.
Sample: Tubulin of BSC-1 cell labeled with Alexa Fluor® 647, acquisition time: 20 seconds -
Large image acquisition area
New intermediate zoom lenses in the imaging system have been developed and optimized for a wide field of view. The wide-view mode achieves 80 μm x 80 μm, a 4-fold increase in imaging area compared to previous models.
Seamless switching between imaging modalities for multi-scale experiments
The N-STORM can be simultaneously combined with a confocal microscope such as the AX/AX R. A desired location in a sample can be specified in a low-magnification/large FOV confocal image and acquired in super-resolution by simply switching the imaging method. Combining a confocal microscope with a super-resolution system can provide a method for gaining larger contextual views of the super-resolution information.
Tailored Hardware Configurations
Choose a fully-automated or a simple motorized TIRF illuminator, the latest sCMOS technology or a proven EMCCD camera. With the modular design of Nikon systems, a flexible and stable platform can be tailored to the demands of single molecule imaging and more.
Objectives designed for N-STORM
Silicone immersion objectives
Silicone immersion objectives use high viscosity silicone oil with a refractive index close to that of live cells as an immersion liquid. Because of this improved refractive index compatibility, these objectives can provide improved photon collection capability and resolution when performing super-resolution imaging deeper into the specimen. They exhibit superior chromatic aberration correction and high transmittance over a broad range of wavelengths.
Oil immersion objectives
These objectives provide the high numerical apertures required for N-STORM imaging. The HP objectives are compatible with the ultrahigh power lasers required for inducing rapid photoswitching of fluorophores. They provide improved axial chromatic aberration correction to achieve the highest level of precision in localization and image alignment for 3D multi-color STORM imaging. The AC-type objectives that support the Auto Correction Collar of the Ti2-E microscope allow precise and easy adjustment of the correction collar.
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